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. 2019 Sep 3;55(10):838–853. doi: 10.1007/s11626-019-00403-x

Figure 3.

Figure 3.

YKL-40 affects migration and invasion in D492 cell lines. a YKL-40 is more highly expressed in D492HER2, compared to D492 and D492M, both at mRNA and protein level. b, c Transient siRNA knockdown of YKL40 reduced the ability of D492HER2 to migrate and invade in vitro (mean ± SD, n = 3). Statistical significance was tested using one-way analysis of variance (ANOVA) and Dunnett’s multiple comparison test (**p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001). d, e Knock-down of YKL-40 in D492HER2 in stable cell lines generated by CRISPR/Cas9 technology reduced the ability of D492HER2 to migrate and invade similarly to transient transfection. f, g Accordingly, overexpression of YKL40 in D492 and D492M using CRISPRa system increased their ability to migrate and invade. Overexpression of YKL-40 was confirmed by immunofluorescence staining (Scale bar = 100 μm) and western blotting. Migration and invasion assay results are shown as average of eight replicates (mean ± SD). Unpaired t test was used to test significance (**p ≤0.01, ***p ≤ 0.001, ****p ≤ 0.0001).