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. 2019 Nov 21;10:1357. doi: 10.3389/fphys.2019.01357

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Copyright © 2019 Buchacker, Mühlfeld, Wrede, Wagner, Beare, McCormick and Grothausmann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(a) Slices of the entire tissue block (525 × 525 μm) in the z-direction: A, artery; ACN, alveolar-capillary network; B, bronchiolus; CT, connective tissue; RBC, red blood cells; L, lymphatic vessel; MA, microatelectasis; SA, saccular airspace; V, vein. (b) Segmentation of the structures of interest (same slice as in a): red = blood vessels, green = airspace, yellow = lymphatic vessels. (c) 3D Surface rendering (525 × 525 × 420 μm) of the segmented structures of interest, air: semi-transparent gray, vasculature: red to blue, lymphatic vessels: yellow. (d) Like (c) without the air surface but with the slice from (a).