Figure 2.
Knockdown of XPF and its effect on cell viability after exposure to cisplatin. (A) XPF and ERCC1 detection and relative quantification by western blot in A549 cells wild type or transduced with shXPF lentivirus. Full-lenght membranes are shown on Supplementary Fig. S6. (B,C) HCR assay with a luciferase plasmid irradiated with 600 J/m2 of UVC or treated with 750 nM of cisplatin, respectively. (D) A dose-response viability curve of A549 or A549 shXPF cell lines treated with increasing concentrations of cisplatin and analyzed after 72 h of treatment by XTT assay. Values are mean ± SEM of three independent experiments (two for the western blot experiments), *P < 0.05, **P < 0.01, ***P < 0.001.