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. 2019 Nov 27;9:17639. doi: 10.1038/s41598-019-54065-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Glutathione production profile and NRF2 expression in lung cancer cells and effects in resistance to cisplatin. (A) Quantification of basal intracellular GSH/GSSG ratio in the lung cancer cell lines. (B) Cellular viability, as determined by XTT assay, in A549 cells treated with cisplatin (5 μM) and BSO (500 μM) for 72 h. (C) Quantification of GCLM and xCT mRNAs expression in lung cancer cells at basal levels, by real time PCR, normalized by GAPDH expression. (D) NRF2 protein level detection and relative quantification by western blot in lung cancer cells. Full-lenght membranes are shown on Supplementary Fig. S7. Values are mean ± SEM of three independent experiments (two for the western blot experiments), *P < 0.05, **P < 0.01, ***P < 0.001.