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. 2019 Nov 27;9:17730. doi: 10.1038/s41598-019-54143-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Proliferating, GFAP negative cells give rise to dNSCs following ablation. (a) Schematic of the experimental paradigm. (b) The neurosphere assay for dNSCs (EFH) (grey bars) performed in control (GFAPtk-) and experimental groups (GFAPtk+) at day 10 (i), day 24 (ii), or day 40 (iii) after onset of ablation. The numbers of YFP+ neurospheres are indicated in yellow bars (n = 6 mice/group/survival time). (c) Colony-forming assay for pNSCs (LIF) (orange bars) performed in control (GFAPtk−) and experimental groups (GFAPtk+) at day 10 (i), day 24 (ii), or day 40 (iii) after the onset of ablation. The numbers of YFP+ colonies are indicated in yellow (n = 6 mice/group for day 10; n = 3 mice/group for day 24; n = 4–6 mice/group for day 40). All data represent mean ± SEM.