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. 2019 Nov 28;51(11):1–13. doi: 10.1038/s12276-019-0336-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Representative confocal fluorescence images of AQP1; M, mouse kidney; H, human graft. Scale bars, 100 μm. b t-SNE dot plots showing clusters of kidney organoid cells generated using either the Takasato (T, top) or Morizane (M, bottom) method, analyzed by single cell RNA-seq. c Confocal optical sections of organoids derived using these protocols or human kidney tissue, using identical laser power and gain settings for AQP1 and LTL channels. Images are representative of three different batches of organoids for each protocol. Scale bars, 50 µm.