Fig. 6. Nephron-like structures in kidney organoid grafts are not fully mature.

a Representative immunohistochemical staining images showing vimentin staining in transplanted kidney organoids harvested at 14 days after transplantation, mouse kidneys with postnatal day 1 and adult mouse kidneys. Magnified portions of the images are shown to the right of the images. White arrows indicate parietal epithelial cells and black arrows indicate tubules. Scale bars, 50 μm. b Representative confocal images of HNA and vimentin in the transplanted kidney organoids at 14 days after transplantation. Scale bars, 50 μm. c Representative confocal images of vimentin in kidney organoid outgrowth cells 7 days after transfer onto glass plates coated with 3% GelTrex. Scale bars, 200 μm.