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. 2019 Nov 27;2:436. doi: 10.1038/s42003-019-0669-y

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© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2019

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Fig. 4 — P53LOH is associated with the switch from homologous recombinational repair (HRR) to nonhomologous end joining (NHEJ) and genomic instability. a Rad51(marker for HRR) IHC in ErbB2 mammary tumors of mice with indicated p53 genotypes. b Ku70 (a marker for NHEJ) in ErbB2 mammary tumors of mice with indicated p53 genotypes. Four mammary tumors per genotype were stained. c H&E staining of normal anaphase showing the segregating masses of chromosomes and bridging (arrow) between the segregating masses of chromosomes during anaphase. d p53 IHC staining in the tumor from flR248Q/−;ErbB2 mouse injected with oil or following the depletion of p53 in the tumor from flR248Q/-;ErbB2 mouse after tamoxifen injection. The scale bars in A–D represent 50 μm. Images in A–D are representative stainings from 10 mice per group. e Quantification of anaphase bridges (AB) in ErbB2 mammary tumors of mice with indicated p53 genotypes. n = 3 tumors per genotype. *p < 0.05; **p < 0.01; ***p < 0.001. f Staining for mitotic spindles in a mitotic cell (metaphase) in p53H/-;ErbB2 mouse mammary tumor (a, e). Nuclear staining (DAPI), (b, f) centrosomes (γ-Tubulin), (c, g) mitotic spindles (α-Tubulin), (d, h) merge. a–d a mitotic cell with normal (2) spindle poles and 2 centrosomes. e–h a mitotic cell with no centrosomes (acentrosomal) and abnormal (>2) spindle poles. Arrows point to the position of the centrosomes in the mitotic cell. Asterisks indicate the three directions of the pull of the acentrosomal spindle poles. Data are representative of 10 images from 4 mice per genotype. The scale bar represents 145 μm.