Table 2.
Amplification efficiencies (E) and correlation coefficients (R2) obtained for primers used to amplify reference candidate genes in samples of F. varia, M. quadrifasciata and S. bipunctata. The annealing temperature was 60 °C for all primers and reactions.
| Gene | F. varia | M. quadrifasciata | S. bipunctata | |||
|---|---|---|---|---|---|---|
| E (%) | R2 | E (%) | R2 | E (%) | R2 | |
| act | 115.0 | 0.700 | 112.7 | 0.700 | 160.4 | 0.924 |
| ef1-α | 98.2 | 0.996 | 101.0 | 0.998 | 91.5 | 0.997 |
| gapdh | 97.0 | 0.997 | 95.2 | 0.997 | 90.2 | 0.996 |
| rpl32 | 98.9 | 0.998 | 95.5 | 0.993 | 93.8 | 0.994 |
| rps5 | 96.1 | 1.000 | 100.3 | 0.996 | 109.8 | 0.992 |
| rps18 | 101.3 | 0.999 | 96.9 | 0.995 | 95.4 | 0.995 |
| tbp | 312.3 | 0.994 | 218.9 | 0.880 | 257.9 | 0.774 |
| tbp-af | 99.7 | 0.992 | 96.8 | 0.996 | 92.9 | 0.994 |