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. 2019 Nov 27;10(12):898. doi: 10.1038/s41419-019-2133-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Schematic representation of the use of adeno-associated viral vectors encoding rLmx1a, rNurr1 and rPitx3. b Representative fluorescence images of immunoreactivity for the dopaminergic neuronal marker TH (red) and the pan-neuronal marker β-Tubulin (green) at DIV 20. Nuclei are counterstained with DAPI (blue). Scale bars: 100 µm. c Quantitative analysis of total percentage of neurons obtained after viral vector-mediated patterning. Data represent the average percentage of total neurons of all cells in culture (β-Tubulin⁺/DAPI⁺). d Quantitative analysis of total percentage of dopaminergic neurons obtained after viral vector-mediated patterning. Data represent the average percentage of total dopaminergic neurons out of total number of neurons in culture (TH⁺/β-Tubulin⁺). e Quantitative analysis of total percentage of dopaminergic neurons obtained after viral vector-mediated patterning. Data represent the average percentage of total dopaminergic neurons out of total number of cells in culture (TH⁺/DAPI⁺). In all graphs, bars represent the average percentage ± SD from three independent differentiations and independent transductions for each of the four hiPSC lines. Non-significant (n.s.) via two-way ANOVA followed by Bonferroni’s post-hoc test.