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. 2019 Nov 27;10(12):898. doi: 10.1038/s41419-019-2133-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Experimental design to monitor expression of EGFP in neurons obtained after rLmx1a viral vector-mediated patterning. b, c Representative live cell images showing EGFP expression (c) or lack of (b) in hiPSC-derived neurons at DIV 25 (c) vs DIV 20 (b). Scale bar: 100 µm. d, e Quantitative analysis of endogenous spontaneous activity; synchronous (d) or asynchronous (e) activity observed after transducing CT-01 hiPSC-derived neurons with AAV-hSyn1-GCaMP3.5-EGFP. Data represented as change in average intensity (ΔF/F0 of GCaMP3.5-EGFP fluorescence) recorded over 25 s per visual field.