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. 2019 Nov 27;9:17727. doi: 10.1038/s41598-019-54177-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Down-regulation of Sema4C expression reverses EMT in cervical cancer cells. (A,C,D) Immune-histochemical method and RT-PCR were used to detect the up-regulation of Sema4C in cervical cancer tissues. Data are presented as mean ± SD. P = 0.0141, n = 52. (B) Patients with high Sema4C expression levels had shorter OS and DFS than those with low expression levels of Sema4C. Data are presented as mean ± SD. P values for overall survival (OS) and disease-free survival (DFS) were evaluated with Kaplan-Meier survival curves and were compared by the log-rank test. **P = 0.008, n = 26. (E) Western blot showed that the up-regulation of Sema4C in four CC cell lines Hela, Siha, Caski and C33a, C5 as the positive control. Data are presented as mean ± SD. n = 3. (F) Western blot showed that down-regulation of Sema4C in Caski cells of cervical cancer resulted in increased expression of epithelial markers E-cadhein, while decreased expression of interstitial markers Vimentin and EMT-related transcription factor Snail. (G) Graphic representation of Sema4C expression in the four CC cell lines. (H) Graphic representation of relative expression of EMT proteins in the Sema4C treated Caski cells. Data are presented as mean ± SD. ^##P = 0.022, n = 3.