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. 2019 Nov 27;9:17727. doi: 10.1038/s41598-019-54177-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overexpression of miR-31-3p reverses EMT and inhibits cell migration and invasion in cervical cancer cells. Caski cells were transfected with miR-31-3p mimic or negative control mimic. (A) After 24 hours of transfection, the expression level of miR-31-3p in Caski cells was detected by real time PCR. Data are presented as mean ± SD, Paired student’s t test was analyzed. ^#P = 0.0061, n = 3. (B,C) Expression levels of Sema4C, E-cadherin, Vimentin and Snail proteins in Caski cells were detected by western blotting after 48-hour transfection. Data are presented as mean ± SD. P value was analyzed by simple one-way ANOVA analysis, ^#P < 0.05, n = 3. (D–G) After 24 hours of transfection, cell invasion assay and wound healing assay were used to detect the ability of cell migration and invasion. GAPDH is the internal references. Data are presented as mean ± SD, n = 3.