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. 2019 Nov 27;9:17675. doi: 10.1038/s41598-019-53240-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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IL-23 induced inflammation drives accumulation of Tregs. Animals received four daily intradermal (ear) injections of vehicle or IL-23 (days 0–3) and were analyzed on day 4. (A) Measurement of ear thickness. (B) Total cellularity and number of CD45⁺ cells in the ear. (C) Representative flow cytometry plots of live CD45⁺CD4⁺TCRβ⁺ cells in the ear. (D) Frequency and number of Foxp3⁺ (live CD45⁺CD4⁺TCRβ⁺Foxp3⁺ cells) in the ear of mice. (E and G) Representative flow cytometry plots of Foxp3⁺ cells in the ear. (F and H) Frequency and number of the indicated cell populations in the ear. (A) Representative data of at least 3 independent experiments, n = 4. (B–F) Pooled data from two independent experiments, n = 8. (G and H) Data represents pooled analysis of 2 vehicle or IL-23 treated ears. Representative data from two independent experiment, at least n = 3 in each experiment. Live CD45⁺CD4⁺TCRβ⁺Foxp3⁺ cells in the ear are defined as Foxp3⁺ cells in the figure. *p < 0.05 determined by student’s t test.