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. 2019 Nov 27;9:17675. doi: 10.1038/s41598-019-53240-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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IL-23 induces a population of Th17 like Tregs that is also sensitive to RORγt inhibition. Vehicle or IL-23 treated ears were analyzed on day 4. (A) Representative flow cytometry plots of live CD45⁺CD4⁺TCRβ⁺IL-17A⁺ cells in the ear. (B) Frequency of live CD45⁺CD4⁺TCRβ⁺IL-17A⁺RORγt⁺Foxp3⁺cells in the ear. Data represents pooled analysis of 2 vehicle or IL-23 treated ears. Representative data from two independent experiments, at least n = 3 in each experiment. (C) Absolute ear thickness and (D) area under the curve (AUC) measurement in mice treated with vehicle or IL-23 in the presence or absence of a RORγt(i) at 100 mg/kg PO-QD. (E) Representative flow cytometry plots and (F) frequency of Th17-like Tregs cells in the draining lymph nodes of mice treated with IL-23 and either vehicle or 100 mg/kg of a RORγt(i) PO-QD. Live CD45⁺CD4⁺TCRβ⁺Foxp3⁺ cells in the ear and live CD4⁺TCRβ⁺Foxp3⁺ cells in the draining lymph nodes are defined as Foxp3⁺ cells in the figure. Data represents a study of n = 6 per group, with similar results on efficacy of RORγt(i) in IL-23 treated animals obtained from independent studies with other RORγt(i) compounds. *p < 0.05 determined by student’s t test.