Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 27;9:17663. doi: 10.1038/s41598-019-54184-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

KDM5B is serine phosphorylated by CDK1. MDA-MB-231 cells were infected with control shRNA lentiviruses or CDK1 shRNA lentiviruses. (a) Lysates from MDA-MB-231 cells were immunoprecipitated using antibodies against phosphorylated serine (pS) and analyzed for KDM5B by Western blotting. (b) MDA-MB-231 cells were treated with kinase inhibitors (Erk inhibitor, Vx: Vx-11e, 100 μM or CDK1 inhibitor, RO: RO3306, 10 μM) and lysates were immunoprecipitated using antibodies against KDM5B and analyzed for phosphorylated serine by Western blotting. (c) Lysates from MDA-MB-231 cells were immunoprecipitated using antibodies against KDM5B and CDK1 and analyzed for co-immunoprecipitation of CDK1 and KDM5B, respectively, by Western blotting. Normal rabbit IgG was used as a negative control. Input lanes represent 25% of the total protein. (d) Upper panel: In vitro kinase assay wherein recombinant cyclin B1 were incubated with purified GST-KDM5B in the absence or presence of CDK1 or ATP. Phosphoserine signal was detected by Western blotting. Lower panel: Western blot analyses of purified GST-KDM5B used in kinase assays. Figures are representative of at least 3 independent experiments.