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. 2019 Nov 27;9:17663. doi: 10.1038/s41598-019-54184-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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KDM5B mediates expression of pluripotency genes and role of CDK1 in KDM5B action. TNBC cells were transfected as indicated above with expression vectors for WT or mutant FLAG-KDM5B, shKDM5B, and/or shCDK1. Two clones each of shKDM5B (14758 and 14761) and shCDK1 (585 and 603) were used. (a) RNA was isolated and subjected to qRT-PCR to assess SOX2 and NANOG mRNA levels using GAPDH as a control. (b–d) Western blotting analyses of endogenous and/or transfected KDM5B, CDK1, Nanog, and/or Sox2 relative to loading control (GAPDH) in TNBC cell lines. Figures are representative of at least 3 independent experiments.