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. 2019 Nov 27;9:17663. doi: 10.1038/s41598-019-54184-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HEXIM1 counteracts is required for the inhibitory effects of KDM5B. MDA-MB-231 cells were transfected with expression vector for shHEXIM1, shKDM5B, and/or FLAG-HEXIM1. (a) Lysates were analyzed by Western blotting using indicated antibodies. (b) RNA was isolated and subjected to qRT-PCR or lysates were analyzed by Western blotting for expression of indicated genes or proteins, respectively. (c) RNA was isolated and subjected to qRT-PCR analyses of SOX2 and NANOG mRNA levels using GAPDH as a control. Figures are representative of at least 3 independent experiments.