Figure 4.

Distribution and density of Iba1-positive macrophages in each part of the cochlea of wild type and Csf1^op/op mice. Iba1-positive macrophages are found in the spiral ganglion (A,D), spiral ligament (B,E), and intermediate cell layer of the stria vascularis (C,F) in both wild type (WT) and Csf1^op/op mice at the age of 4 weeks. While there is no statistically significant difference in the density of Iba1-positive macrophages in the spiral ganglion, a significant reduction in the density of Iba1-positive cells is observed in Csf1^op/op mice, suggesting Csf1 dependency of resident macrophages in the spiral ligament and stria vascularis (G, single plots are also shown; n = 4 for each genotype). Bar: 200 μm for (A–F) (shown only in D). *p < 0.05.