Figure 1.

Inhibitory activity of FAM E3 on ZIKV replication. Schematic representation of ZIKV-Nanoluc that continuously expresses the Nanoluciferase reporter (a). Chemical structure of FAM E3 (b). Dose response assay: ZIKV-Nanoluc infected cells (MOI 0.1) were treated with FAM E3 at concentrations ranging from 1 to 10 µM and virus replication efficiency was evaluated 72 h.p.i. Simultaneously, Vero cells were equally treated with FAM E3 and cells viability was measured 72 h later (c). Effective and cytotoxic concentration of 50%: Vero cells were treated with increasing concentrations of FAM E3 ranging from 0.10 to 200 µM. ZIKV replication was measured by luciferase assay (indicated by ■) and cellular viability measured using an MTT assay (indicated by ●) (d). Vero cells were infected with ZIKV^BR and treated with FAM E3 at 3 µM and virus replication was accessed 72 h.p.i. The intracellular virus was titrated by analysing focus- forming units per milliliters (Ffu/mL), DMSO and OLX were used as negative and positive controls, respectively (e). Huh-7 or 293 T cell lines were infected with ZIKV-Nanoluc and treated with FAM E3 (3 µM) or DMSO (0.1%) for 72 h (f). Mean values of three independent experiments each measured in quadruplicate including the standard deviation are shown. P < 0.05 was considered significant compared to DMSO control.