Figure 2.

Effects of FAM E3 on the different stages of the ZIKV replicative cycle. Vero cells were infected with ZIKV-Nanoluc at a MOI = 0.5 and simultaneously treated with FAM E3 for 2 h; cells were washed to remove the virus and replaced with fresh media. ZIKV replication was measured by Nanoluc activity at 72 h.p.i (a). Vero cells were treated with FAM E3 for 2 h. Then, cells were extensively washed and infected with ZIKV-Nanoluc at a MOI = 0.5 for 2 h. The inoculum was removed and the cells were washed and replaced with fresh media. ZIKV replication was measured by Nanoluc activity at 72 h.p.i. (b). Vero cells were infected with ZIKV-Nanoluc at a MOI = 0.5 for 2 h. The virus was removed, cells were washed and added of fresh media containing FAM E3. ZIKV replication was measured by Nanoluc activity at 72 h.p.i (c). For all assays, non-infected Vero cells were equally treated with FAM E3 and cell viability was measured 72 h later using MTT assay. DMSO was used as negative control and OLX as positive control for infectivity inhibition. Mean values of three independent experiments each measured in quadruplicate including the standard deviation are shown. P < 0.05 was considered significant.