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. 2019 Nov 27;9:17703. doi: 10.1038/s41598-019-54169-z

Figure 3.

Figure 3

Analysis of FAM E3 intercalation into the viral dsRNA and its interaction with the activity of phage SP6 RNA polymerase. Fifteen nanomoles of dsRNA were incubated with the FAM E3 or intercalating controls (DMSO) or (DOX) for 45 minutes at room temperature. The reaction products were subjected to 1% agarose electrophoresis gel containing Ethidium Bromide followed by densitometry analysis (a). FAM E3 and 5 µg of purified pCCI-SP6-ZIKV amplicon was used for in vitro transcription using SP6 RNA polymerase at the presence or absence of FAM E3. Reaction products were analysed by agarose gel electrophoresis followed by densitometry analysis (b). Results of a representative of three independent reproducible experiments are shown.