Figure 5.
The Zn2+-induced [Ca2+]i increase is PLC-β-dependent. Fura-2-loaded HSG cells were treated with 30 µM ZnCl2 (a), 300 µM carbachol (b), or 100 µM histamine (c) after pre-incubation with vehicle (left), 3 µM U73122 (middle), or 20 µM 2APB (left). Quantification of [Ca2+]i increase inhibition mediated by carbachol or ZnCl2 was normalized to vehicle groups. The experiments were performed three times independently, and the results were reproducible. Numbers of data were indicated inside bars. **P < 0.01, ***P < 0.001, compared with vehicle control.