Figure 5.

The Zn²⁺-induced [Ca²⁺]_i increase is PLC-β-dependent. Fura-2-loaded HSG cells were treated with 30 µM ZnCl₂ (a), 300 µM carbachol (b), or 100 µM histamine (c) after pre-incubation with vehicle (left), 3 µM U73122 (middle), or 20 µM 2APB (left). Quantification of [Ca²⁺]_i increase inhibition mediated by carbachol or ZnCl₂ was normalized to vehicle groups. The experiments were performed three times independently, and the results were reproducible. Numbers of data were indicated inside bars. **P < 0.01, ***P < 0.001, compared with vehicle control.