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. 2019 Nov 27;9:17668. doi: 10.1038/s41598-019-54087-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of lymphocyte markers on effector T-lymphocytes after activation with DCs pulsed with antigens. Autologous lymphocytes (non-adhering PBMCs) were activated to become effector T-lymphocytes by co-culturing with DCs pulsed with cell lysate or RNA prepared from single, double, or triple HCC cell lines for 2 days. Effector T-lymphocytes were then further propagated in AIM-V medium supplemented with IL2, IL7, and IL15 for 10 days. The expressions of CD3+, CD4+, CD8+, and CD56+ were examined by flow cytometry after staining with specified antibodies. The percentages (mean ± SEM) of specific populations of effector cells, including CD3+ CD4+ helper T-cells (A), CD3+ CD8+ cytotoxic T-cells (B), CD3-CD56+ NK cells (C), and CD3+ CD56+ NKT cells (D) were calculated from three independent experiments. (*p < 0.05 compared with non-adhering PBMCs. Data analyzed by one-way ANOVA with Tukey’s correction).