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. 2019 Nov 27;9:17668. doi: 10.1038/s41598-019-54087-0

Figure 3.

Figure 3

IFN-γ production profile of helper T-cells and cytotoxic T-cells in effector T-lymphocytes. After activation and propagation for 10 days, effector T-lymphocytes were treated with PMA and ionomycin for 24 hours, and then investigated for intracellular IFNγ production by flow cytometry. Effector T-cells were primarily gated by CD3+ cells, and then the IFNγ production profile of CD4 + helper T-cells (A) and CD8+ cytotoxic T-cells (C) was further analyzed. Data of CD4+ IFNγ+ (B) and CD8+ IFNγ+ (D) populations are shown as mean ± SEM, which were calculated from three independent experiments. (*p < 0.05 compared with non-adhering PBMCs. Data analyzed by one-way ANOVA with Tukey’s correction).