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. 2019 Nov 27;9:17651. doi: 10.1038/s41598-019-54163-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Specificity of the SCAR marker. Lane 1, 26-D2000 DNA Marker, lanes 2–6: Tilletia laevis, lane 7–11: Tilletia controversa, lanes 12–15: Tilletia tritici; lanes 16–18: Puccinia striiformis f. sp. tritici, lanes 19–21: Puccinia triticina, lanes 22–25 and 27: Ustilago maydis, lanes 28–32: Ustilago horde, lanes 33–37: Blumeria graminis, lanes 38–42: Fusarium graminearum, lanes 43–47: Ustilago nuda, lanes 48–50: ddH₂O.