Table 3.

The PUFA (polyunsaturated fatty acid) cohort in dry and imbibed M. truncatula seeds. For each representative component, concentration is expressed as percentage (%) of the total lipid content.

Treament	Linolelaidic acid (trans - 18:2)	Linoleic acid (18:2, ω - 6)	γ-Linolenic acid (18:3, ω - 6)	Dihomo γ-linolenic acid (20:3, ω - 6)	α-Linolenic acid (18:3, ω - 3)	Docosahexaenoic acid (22:6, ω - 3)
DS	0.08 ± 0.03	29.67 ± 0.31	0.10 ± 0.01	0.10 ± 0.03	25.80 ± 1.05	0.60 ± 0.01
Imbibition
0.5 h	0.16 ± 0.09	29.53 ± 0.93	0.16 ± 0.07	0.18 ± 0.08	23.08 ± 0.33	0.75 ± 0.13
2 h	0.16 ± 0.10	28.59 ± 0.82	0.19 ± 0.15	0.21 ± 0.06	26.44 ± 3.21	0.87 ± 0.25*
4 h	0.10 ± 0.08	29.58 ± 0.45	0.13 ± 0.06	0.13 ± 0.03	24.38 ± 0.42	0.71 ± 0.14
6 h	0.06 ± 0.01	28.88 ± 0.25**	0.11 ± 0.03	0.10 ± 0.04	23.75 ± 0.50	0.72 ± 0.11
8 h	0.09 ± 0.02	29.43 ± 0.85	0.20 ± 0.05*	0.10 ± 0.06	24.38 ± 1.53	0.67 ± 0.31

Values are the mean ± SD of three independent replications with 20 seeds for each replication. Asterisks indicate statistically significant differences determined using Student’s t-test (* P < 0.05; ** P < 0.01). DS, dry seed. n.d., not detected.