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. Author manuscript; available in PMC: 2020 May 15.
Published in final edited form as: Cancer Res. 2019 Sep 13;79(22):5711–5722. doi: 10.1158/0008-5472.CAN-19-1034

Figure 1. MUC1-C drives MTA1, MBD3 and CHD4, and not HDAC1, expression.

Figure 1.

A-D. BT-549 (A,B) and SUM149 (C,D) cells stably expressing a tet-CshRNA or tet-MUC1shRNA were left untreated or treated with 500 ng/ml DOX for 7 days. Cells were analyzed for MUC1-C, MTA1, MBD3, CHD4 and HDAC1 mRNA levels using primers listed in Supplemental Table S1. The results (mean±SD) are expressed as relative mRNA levels compared to that obtained for untreated cells (assigned a value of 1). The asterisk (*) denotes a p-value of <0.05. E-F. Lysates were immunoblotted with antibodies against the indicated proteins.