A. RNA-seq was performed in triplicate on BT-549/CshRNA and BT-549/MUC1shRNA cells. The BT-549 datasets were analyzed by GSEA, using the Hallmark gene signature collection. Silencing MUC1-C expression was significantly associated with induction of the Estrogen Response Early and Estrogen Response Late gene sets. B-E. BT-549 cells expressing a CshRNA, MUC1shRNA (B), MTA1shRNA (C), MBD3shRNA (D) or CHD4shRNA (E) were analyzed for the indicated mRNA levels. The results (mean±SD) are expressed as relative mRNA levels compared to that obtained for CshRNA expressing cells (assigned a value of 1). F. Normalized, log2 transformed expression data were obtained for the TCGA-BRCA cohort and correlations were determined. Pearson correlation coefficient and significance are shown.