Figure 1:

Acute LAN Increases Depressive-like Behavior (A-C) Average time spent floating, number of floating bouts, and latency to float during forced swim test. (A) LAN significantly increased floating duration in females relative to LD (t= 3.133; p<0.008; Bonferroni correction)(F LD n=15; M LD n= 16; F LAN n=16; M LAN n=14). (B) Main effect of lighting and sex on the number of floating bouts (F_1,58=20.63, p<0.001; F_1,58=7.568, p<0.01); increased number of floating bouts in males and females housed in LAN relative to sex matched controls. (F LD n=15; M LD n= 16; F LAN n=16; M LAN n=15) (C) No group differences in latency to float (p>0.008 for all t-tests; Bonferroni correction) (F LD n=15; M LD n= 15; F LAN n=16; M LAN n=14). (D-E) Percent sucrose preference and total liquid consumption during sucrose preference testing. (D) LAN significantly decreased sucrose preference in males and female mice relative to their sex matched controls (males, t=3.893; p<0.001; females, t=5.043; p<0.0001; Bonferroni correction)) (F LD n=14; M LD n= 14; F LAN n=14; M LAN n=15) (E) LAN reduced total liquid consumption in females relative to males house in normal lighting condition (t=3.320; p<0.008; Bonferroni correction). No differences in total liquid consumption were detected within sexes (p>0.008 for all t-tests; Bonferroni correction) (F LD n=14; M LD n= 14; F LAN n=15; M LAN n=15) Error bars represent SEM; # main effect of lighting, * main effect of sex, + sex by lighting interaction; (B) two-way ANOVA; Tukey’s multiple comparisons test (A and C-E) multiple t-tests between each group with Bonferroni correction. Bars that do not share a letter represent Tukey’s multiple comparisons at p<0.05 or Bonferroni correction at p<0.008.