Figure 4:
Acute LAN Alters Activity and Body Temperature Rhythms (A-B) Activity rhythms in female and male mice exposed to LAN; 2 hr bins beginning at ZT6 on Day 1 and ending at ZT4 on Day 4. (C) Graphic illustrating the phi (phase) of activity rhythms for each group from CT0 (D) Main effect of lighting (F1,28=12.41, p<0.01) on tau (period) of activity rhythms. (F LD n=7; M LD n= 9; F LAN n=8; M LAN n=8) (E) Main effect of lighting (F1,25=48.16, p<0.0001), sex (F1,25=21.51, p<0.0001), and sex by lighting interaction (F1,25=11.24, p<0.01) on phi of activity rhythms; LAN increased phi in females relative to same sex controls. (F LD n=7; M LD n= 8; F LAN n=7; M LAN n=7) (F) No group differences in the amplitude of activity rhythms (F LD n=7; M LD n= 9; F LAN n=7; M LAN n=8) (G-H) Body temperature rhythms in female and male mice exposed to LAN; 2 hr bins beginning at ZT6 on Day 1 and ending at ZT4 on Day 4. (I) Graphic illustrating the phi (phase) of body temperature rhythms for each group from CT0 (J) Main effect of lighting (F1,28=12.41, p<0.01) on tau (period) of body temperature rhythms. (F LD n=7; M LD n= 9; F LAN n=8; M LAN n=8) (K) Main effect of lighting (F1,26=15.59, p<0.001) on phi (phase) of body temperature rhythms; female mice exposed to LAN demonstrate increased phi compared to female mice housed in LD. (F LD n=7; M LD n= 8; F LAN n=8; M LAN n=7) (L) Main effect of lighting (F1,28=10.23, p<0.01) and sex (F1,28=41.16, p<0.0001) on the amplitude of body temperature rhythms; LAN reduced the amplitude of body temperature rhythms in male mice relative to sex matched controls. (F LD n=7; M LD n= 9; F LAN n=8; M LAN n=8) Error bars represent SEM; # main effect of lighting, * main effect of sex, + sex by lighting interaction; bars that do not share a letter represent multiple comparisons at p<0.05, two-way ANOVA; Tukey’s multiple comparisons test.