Figure 2.

Murine and human cancers increase circulating pre-B-like cells, as revealed by FACS phenotyping in the indicated tissues and primary tumors of BALB/c mice with 4T1 cancer (n=14–32; shown frequency, A, and absolute numbers, B, of CD25⁺ Pre-B-like cells ) and C57BL/6 mice with spontaneous ovarian cancer (n=6–13, mogp, C). Mice with EMT6 cells, despite having a larger tumor than 4T1 cells (D), inefficiently increased CD25⁺pre-B-like cells (E, n=5, the result was reproduced twice). Compared with healthy people (HD, n=18), peripheral blood of humans with breast cancer markedly increased the frequency of CD10⁺CD19⁺ B cells (BC, n=85, F). RT/PCR assay confirmed expression of RAG1, RAG2, and VPREB in tumor infiltrating B cells in humans with BC (TIL-B, n=5, relative to GAPDH, G). Controls were tonsils (n=2, G). A–F, each symbol is for a single mouse or human and G shows mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001; ns, not significant in Mann-Whitney Wilcoxon t-test (A–F).