Figure 3.

Hematoxylin-eosin staining and immunohistochemical staining on paraffin-embedded tissue sections showed the morphology of the vessels and the upregulation of ET_A and ET_B after LPS treatment, respectively. Arteries (without endothelium) were taken from rats treated with NS or LPS (5 mg/kg body weight) for 6 hrs. The arrowhead points to the positive staining of ET_A or ET_B protein. L: lumen. The size bar corresponds to 200 μm (150x) or 50 μm (600x. (b, c) Semiquantitation of immunohistochemistry results by using ImageJ software. Data are expressed as mean ± SD. Unpaired Student's t-test, ^∗∗∗∗P < 0.0001 versus NS, n = 10 sections (2 sections per animal) in each group.