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. 2019 Nov 13;2019:6279313. doi: 10.1155/2019/6279313

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Copyright © 2019 Md. Ataur Rahman et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effects of OGA and OGT knockdown on autophagy in astrocytes. (a) Astrocytes were transfected with OGA and OGT siRNA for 48 h. Immunoblot showing OGA, OGT, and O-GlcNAcylation levels. (b) Dot blot analysis of O-GlcNAcylation in cells transfected with OGA or OGT siRNA (n = 3, ^∗p < 0.05, ^∗∗∗p < 0.001). (c) Immunofluorescence staining of astrocytes was performed by astrocyte-specific anti-GFAP and anti-LC3 antibodies via confocal microscopy. (d) The number of LC3 puncta was counted and analyzed by ±SEM (^∗∗∗p < 0.001, ns = nonsignificant). (e) Immunoblot was indicated by LC3 and p62 expression of OGA- or OGT siRNA-treated cells (n = 3, ^∗∗p < 0.01).