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. 2019 Aug 29;9(1):61–78. doi: 10.1016/j.jcmgh.2019.08.008

Figure 2.

Figure 2

miR-148a expression in normal mouse stomach. (A) Fluorescence in situ hybridization for miR-148a (red) and immunofluorescence staining for GSII (green) and DAPI (blue). miR-148a was strongly expressed in the base of corpus glands deep to GSII-positive mucous neck cells. miR-148a expression was localized in the cytoplasm. No or little expression of miR-148a was seen in antrum and duodenum. (B) Fluorescence in situ hybridization for miR-148a (red) and immunofluorescence staining for GSII (blue) and Mist1 or GIF (green). miR-148a–positive cells expressed Mist1 and GIF. (C) Fluorescence in situ hybridization for miR-148a using Mist1CreERT2/+;R26RtdTomato/+ mice. miR-148a (green), tdTomato (red), and GSII (blue). miR-148a was expressed in tdTomato-positive chief cells.