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. 2019 Aug 29;9(1):61–78. doi: 10.1016/j.jcmgh.2019.08.008

Figure 3.

Figure 3

miR-148a expression during chief cell transdifferentiation into SPEM cells. (A) Fluorescence in situ hybridization for miR-148a (red) and immunofluorescence staining for GSII (green) and DAPI (blue). miR-148a was significantly down-regulated in GSII-positive SPEM cells developed in mice after 10 days of DMP-777 treatment or 3 days of L635 treatment as well as in chief cells without GSII expression after 3 days of DMP-777 treatment or 1 day of L635 treatment, compared with normal chief cells. (B) Quantitation of relative miR-148a staining intensity in chief cells and transdifferentiating chief cells at the base of the gland. One-way analysis of variance, P < .0001. Bonferroni multiple comparisons, ****P < .0001. (C) Fluorescence in situ hybridization for miR-148a (red) and immunofluorescence staining for GSII (green) and DAPI (blue). miR-148a was significantly decreased in GSII-positive SPEM cells developed in mice 6 months or 12 months after H felis infection. (D) Quantitation of relative miR-148a staining intensity in chief cells and H felis infection induced SPEM cells at the base of the gland. One-way analysis of variance, P = .0081. Bonferroni multiple comparisons, *P < .05, **P < .01.