Figure 5.
Involvement of miR-148a in initiation of chief cell transdifferentiation. (A) Expression of miR-148a in ImChief cells and ImSPEM cells by microRNA assay. Mann-Whitney U test, *P < .05. (B) Expression of miR-148a by microRNA assay in ImChief cells treated with control inhibitor or miR-148a inhibitor. Mann-Whitney U test, *P < .05. (C) Expression of genes related with SPEM cells (He4, Sox9, Cd44v9, and Clu) or chief cells (Pgc and Mist1) in ImChief cells treated with control inhibitor or miR-148a inhibitor by quantitative PCR. Mann-Whitney U test, *P < .05. (D) Expression of miR-148a target genes in ImChief cells treated with control inhibitor or miR-148a inhibitor by quantitative PCR. Mann-Whitney U test, *P < .05.