. 2019 Nov 22;5(11):e02891. doi: 10.1016/j.heliyon.2019.e02891

Table 6.

Inhibitory activity of the neutralized, heat and proteinase K treated supernatants from Bifidobacterium longum BB536 and Lactobacillus rhamnosus HN001 grown alone against indicator strains using the agar diffusion method (ADM).

Bacterial reference strains	Treated supernatants^#
Bacterial reference strains	nBB536-CFS	nHN001-CFS	htBB536-CFS	htHN001-CFS	pkBB536-CFS	pkHN001-CFS
Escherichia coli ATCC 25922	++	++	+	+	+	+
Escherichia coli ATCC 35218	++	++	++	+	+	+
Klebsiella pneumoniae ATCC 700603	++	++	++	++	+	+
Pseudomonas aeruginosa ATCC 27853	++	++	++	+	+	+
Enterococcus faecalis ATCC 29212	+++	++	++	++	+	+
Staphylococcus aureus ATCC 29213	++	++	++	++	+	+

Neutralized (pH = 7.0) cell-free supernatant from Bifidobacterium longum BB536 (nBB536-CFS) and Lactobacillus rhamnosus HN001 (nHN001-CFS); heat treated (121 °C, 15 min) cell-free supernatant from Bifidobacterium longum BB536 (htBB536-CFS) and Lactobacillus rhamnosus HN001 (htHN001-CFS); proteinase K treated cell-free supernatant from Bifidobacterium longum BB536 (pkBB536-CFS) and Lactobacillus rhamnosus HN001 (pkHN001-CFS). Agar diffusion method (ADM) +++ (very strong): diameter of inhibition zone ≥20.0 mm; ++ (strong): diameter of inhibition zone (20.0, 15.0] mm; + (weak): diameter of inhibition zone (15.0, 10.0] mm; - (no activity): diameter of inhibition zone <10.0 mm; punch diameter = 8.0 mm.