Fig. 3.
Levels of infectious virus and viral RNA at two days after inoculation in JEV-infected mice. Mice inoculated with Mie/41/2002 (n = 5), Muar (n = 5), rJEV-5NCMMuar-M41 (5NCM-Muar) (n = 5), or rJEV-EMuar-M41 (E-Muar) (n = 5) were euthanized at two days after inoculation, and serum, brain, and spleen samples were collected. Sera and tissue homogenates were used to quantify the titer of the infectious virus (PFU/mL or g) (A–C) and the viral genome (genome copies/mL or g) (D–F). Dotted line: detection limit; solid lines: mean values. Significance was analyzed using the Mann–Whitney U test (*p < 0.01).