Characterization of canine mesenchymal stem cells (MSCs).
(A) Canine MSCs. (a) bone marrow mono nuclear cells and (b) fibroblastic morphology in P3. Scale: 100 μm (B) Immunostaining with MSC surface markers and pluripotency markers. Scale: 50 μm. (C) Flow cytometry of surface markers (D) Cytochemical staining after In vitro differentiation into (a) alizarin red, scale: 100 μm (b) alcian blue, scale: 100 μm and (c) Oil red-O, scale: 50 μm.