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Osteogenic differentiation of canine MSCs.
(A) Alizarin red staining over control and CNT films. Scale-100 μm. (B) Immunofluorescence of osteocalcin positive cells. Scale-50 μm. (C) Relative gene expression study. Dashed line indicates values of target genes in control conditions. (D) Flow cytometry of osteocalcin-positive cells. The symbols * and # indicate significance (P < 0.05) with respect to control and between the films, respectively for the qualitative experiments.
