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. 2019 Oct 25;18:966–980. doi: 10.1016/j.omtn.2019.10.019

Figure 9.

Figure 9

circTTN Regulates the Activity of the IGF2/PI3K/AKT Signaling Pathway

(A and D) Bovine primary myoblasts transfected with the PCD2.1-circTTN, IGF2/PI3K/AKT pathway relevant genes (IGF2, IRS1, PI3K, PDK1, and AKT) were detected by quantitative real-time PCR and western blot analysis in proliferation (A) and differentiation (D) stage. (B and E) Bovine primary myoblasts transfected with si-circTTN, IGF2, IRS1, PI3K, PDK1, and AKT were detected by quantitative real-time PCR and western blot analysis in the proliferation (B) and differentiation (E) stage. (C and F) Bovine primary myoblasts were transfected with miR-432 mimic or PCD2.1-circTTN + miR-432 mimic; IGF2, IRS1, PI3K, PDK1, and AKT were detected by quantitative real-time PCR and western blot analysis in proliferation (C) and differentiation (F) stage. β-Actin acts as an internal control gene. Values are means ± SEM for three individuals. *p < 0.05; **p < 0.01.