ASNS is expressed predominantly in pancreatic acinar cells. (A) Immunofluorescence for ASNS (green), amylase (red), and Dolichos biflorus agglutinin-lectin (blue, bottom row) in mouse pancreatic tissue (n = 3). Inset: the white arrows point to amylase within the acinar cells. (B) Relative expression by qPCR of amylase, insulin, and Cytokeratin 19 from isolated ductal (D), islet (I), and acinar (A) cells, to confirm the purity of the isolation method, and (C) qPCR for ASNS in the isolated fractions (n = 2; *P < .05).