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. 2019 Aug 14;9(1):1–13. doi: 10.1016/j.jcmgh.2019.08.003

Figure 4.

Figure 4

Figure 4

Acinar ASNS expression is up-regulated in response to ASNase exposure. With ASNase treatment, there was a (A) concentration- and (B) time-dependent increase in ASNS in 266-6 acinar cells. (C) Transfection of a luciferase construct containing the ATF4 binding site of the ASNS promoter (-173 to +51) in 266-6 cells treated with or without ASNase (5 U/mL; n = 3; *P < .05). (D) Time-dependent increase in ASNS expression in mouse primary acinar cells (n = 2–4; *P < .05, relative to 0 h) and (E) in the rat pancreatic acinar AR42J cell. (F) Increase in ASNS expression in primary human pancreatic acinar cells. (G) In the mouse pancreas by qPCR (left; n = 4; *P < .05) and Western blot (right; n = 3; *P < .05), there was also an increase in ASNS expression with in vivo ASNase exposure. Ctl, control.