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ASNase-induced up-regulation of acinar ASNS is owing to increased transcription, not reduced protein degradation. (A) qPCR for ASNS from 266-6 acinar cells with ASNase exposure for 24 hours (n = 3; *P < .05). (B) Western blot for ASNS from 266-6 cells incubated with CHX (n = 3). (C) An 8-hour CHX chase with or without ASNase exposure showed no difference in ASNS degradation. Densitometry is normalized to 0 hours for each comparison group (n = 3).
