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. 2019 Aug 14;9(1):1–13. doi: 10.1016/j.jcmgh.2019.08.003

Figure 7.

Figure 7

ASNS knockdown provokes pancreatic acinar cell injury and worsens with ASNase exposure, whereas ASNS overexpression protects against ASNase-induced injury. (A) In 266-6 pancreatic acinar cells, ASNase exposure led to a concentration-dependent increase in PI uptake (n = 9–10; *P < .05). (B) Western blot shows knockdown of ASNS in 266-6 acinar cells by shRNA (n = 4). (C) ASNS knockdown induced cell injury in culture over 24 hours, compared with a scrambled control, and knockdown worsened injury with ASNase exposure (n = 10; *P < .05). (D) Western blot shows ASNS plasmid overexpression. (E) Compared with a scrambled control, ASNS overexpression led to reduced PI uptake both at baseline and with ASNase exposure (n = 3; *P < .05). Ctrl, control.