Figure 3.

Estimate fluxes in glucose GNG. A-B, Relative contribution of gluconeogenic substrate and product represented by the ratio of ¹³C enrichment (metabolite versus tracer). C, Estimated fluxes in gluconeogenesis pathway. D, Liver glycogen content. Data are mean ± s.e.m in A, B and D and best-fit value ± 95% confidence interval in C. n = 3 to 4 mice in each group, **P < 0.01; *P < 0.05; ns = not significant by one-way ANOVA. All comparisons are against 6 h data unless indicated otherwise. PDH, pyruvate dehydrogenase; See also Supplementary Fig. 1 and Supplementary Fig. 3.