Identification of AAV-S and AAV-F after Two Rounds of In Vivo Selection for Brain Transduction
Donut charts indicate the frequency of particular peptide inserts determined by NGS. (A) Chart of round 2 vector sequenced after production but before intravenous injection. (B) Chart of peptide frequency appearing in iTransduce isolation after round 2 injection. “Others” indicates sequence variants appearing as less than 1% of the total pool (in A, variants isolated after the round 2 screen are also highlighted, appearing at less than 1%). The asterisk (*) indicates a stop codon.