FIG 5.

Fob1 accumulates at the RFB in the siz2Δ mutant. (a) (i) Wild-type and siz2Δ strains containing 13×Myc-tagged Fob1 (KRY 1802 and KRY 1933, respectively) were grown in YPD, and proteins were extracted. Protein concentration was equalized and loaded after diluting as indicated. Western blotting was done using antibodies (Ant) to Myc to detect Fob1 and antibodies to Pgk1 to confirm equalized protein levels. (ii) Quantification of Fob1 in KRY 1802 and KRY 1933 (n = 3). (b) ChIP using anti-Myc antibodies was performed in wild-type and siz2Δ strains containing 13×Myc-tagged Fob1 (KRY 1802 and KRY 1933, respectively). The levels of enrichment at NTS1 and NTS2 were calculated and plotted as percent input. The graph represents an average from six experiments, and error bars represent SEM. ns, P > 0.05; **, P ≤ 0.01.