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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Electrophoresis. 2018 Dec 27;40(4):571–581. doi: 10.1002/elps.201800417

Figure 1.

Figure 1.

Representative HIC chromatograms of exosome isolation using using PET C-CP fibers. Separations were performed with a mobile phase flow rate of 0.5 mL/min, 60 μL aliquot injections, and a 20 min gradient from 100% buffer A (1.8 M (NH4)2SO4 solution dissolved in PBS; pH = 7.4) to 100% buffer B (30% acetonitrile v/v dissolved in PBS). (A) Baseline chromatogram of pristine HL5 media. (B) D. discoideum-derived EVs previously isolated via differential centrifugation. (C) D. discoideum-derived EVs previously isolated via the Qiagen exoEasy Maxi Kit. (D) D. discoideum-derived EVs following centrifugation to remove cells and large debris. (E) D. discoideum-derived EVs following filtration through a 0.8 μm filter to remove cells and large debris. (F) Abbreviated gradient program (injection at A (0.8 M (NH4)2SO4 solution dissolved in PBS; pH = 7.4), gradient initiated at t = 2 min., gradient to 100% buffer B (30% acetonitrile v/v dissolved in PBS) in 10 min.